Composite

Part:BBa_K322128:Design

Designed by: Chris French and Richard Partridge Hicks   Group: iGEM10_Edinburgh   (2010-10-08)


Phycocyanobilin synthesis genes + Red light detection system (EYFP as reporter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EYFP is used as a reporter system for precise characterisation.


Source

PCR of BBa_M30109 (which is truncated in the 2010 igem distribution).

References